cell lines – Communicable Diseases Research

Identification and characterization of the anti-SARS-CoV-2 activity of cationic amphiphilic steroidal compounds

양이온성 양친매성 스테로이드 화합물의 항 SARS-CoV-2 활성의 확인 및 특성화

Article

Published on 2022-12-012022-09-11
Journal: Virulence
[Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, 치료제,

Ganoderma microsporum immunomodulatory protein acts as a multifunctional broad-spectrum antiviral against SARS-CoV-2 by interfering virus binding to the host cells and spike-mediated cell fusion

Article

Published on 2022-11-012022-11-15
Journal: Biomedicine & pharmacotherapy = Biomédecine & phar
[Category] SARS, 변종, 치료제,

[키워드] ACE2 ACE2-expressing cells acute respiratory syndrome alleviate Antiviral binding blocked Broad-spectrum antiviral against SARS-CoV-2 caused Cell Cell fusion cell lines cell-free virus cells characteristic coronavirus Delta determine Diseases downregulation Effect evaluated examined flow cytometry fluorescence microscopy fluorescent Fungal immunomodulatory proteins fusion GMI HEK293T cell host cell immunomodulatory immunoprecipitation in vitro Infection Infectivity ingredient inhibit inhibited inhibitor Interaction investigated less mechanism multifunctional omicron Protein pseudotyped virus reported SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 pseudovirus SARS-CoV-2 pseudovirus SARS-CoV-2 pseudovirus infection SARS-CoV-2 spike protein SARS-CoV-2 variant spike mutation spike variant spike variants Strains Viral binding. virus were used
[DOI] 10.1016/j.biopha.2022.113766 PMC 바로가기

SARS-CoV-2 infection and replication kinetics in different human cell types: The role of autophagy, cellular metabolism and ACE2 expression

Article

Published on 2022-11-012022-11-16
Journal: Life sciences
[Category] SARS, 유전자 메커니즘,

[키워드] A549 ACE2 ACE2 expression angiotensin ATP synthase autophagy BEAS-2B Brain Caco-2 can be used Cell cell line cell lines cellular cellular metabolism COVID-19 COX endothelial energy metabolism evaluated expression level HEK-293 human cell human cell line in vitro intestine intrinsically investigated kidney liver mechanism metabolism Mitochondria mitochondrial metabolism modified cell line molecular mechanism mRNA expression NDUFS2 overexpressed overexpressing overexpression Protein Replication replication kinetics respiratory tract RT-qPCR SARS-COV-2 infection SARS-CoV-2 replication SH-SY5Y susceptibility susceptible cell TMPRSS2 TMPRSS2 expression TMPRSS2. uninfected cell various tissue viral infection Viral load viral replication were assessed western blot
[DOI] 10.1016/j.lfs.2022.120930 PMC 바로가기

Choosing a cellular model to study SARS-CoV-2

Article

Published on 2022-10-212022-11-15
Journal: Frontiers in Cellular and Infection Microbiology
[Category] COVID19(2023년), SARS, 치료기술, 치료제,

Structural interactions of phytoconstituent(s) from cinnamon, bay leaf, oregano, and parsley with SARS-CoV-2 nucleocapsid protein: A comparative assessment for development of potential antiviral nutraceuticals

Article

Published on 2022-10-012022-11-15
Journal: Journal of food biochemistry
[Category] COVID19(2023년), SARS, 진단, 치료제,

[키워드] affinity Antiviral antiviral agent apigenin binding binding domain binding interaction biological activity C-terminal Cancer Cancer cells candidate carried cell line cell lines cells Cinnamomum cinnamon complex Complexes components Compound computational analysis computational approach CTD cytotoxicity death drug candidate Druglikeness effective Efficacy evaluate evaluated examined greater HPLC in silico in vitro in vivo Interaction lack less molecular molecular dynamics MTT N-terminal Naturally NTD nucleocapsid nutraceutical oregano Origanum outcome parsley. plant predicted profile Prophylactic Protein provided Research responsible RNA SARS-CoV-2 SARS-CoV-2 nucleocapsid SARS-CoV-2 nucleocapsid protein stability symptomatic targets therapeutic therapeutic drug therapy Toxicity treating COVID-19 Treatment virus was determined was performed worsened
[DOI] 10.1111/jfbc.14262 PMC 바로가기

SARS-CoV-2 modulates virus receptor expression in placenta and can induce trophoblast fusion, inflammation and endothelial permeability

Article

Published on 2022-09-132022-11-16
Journal: Frontiers in Immunology
[Category] SARS, 유전자 메커니즘,

[키워드] ACE2 addition Apoptosis CD147 Cell cell lines cells Coagulation complement Cytokine storm Effect elevated elicit endothelial event Evidence expression fetal Hyperactivation immunopathological Impact incidence increased vascular permeability induce infected with SARS-CoV-2 Infection Inflammation less main receptor mechanism modulate Mortality mother outcomes pathway Placenta placental positive Pregnancy pregnancy. pregnant Preterm birth pro-inflammatory cytokine Protein raise SARS-CoV-2 spike susceptibility syndrome the SARS-CoV-2 TMPRSS2 TMPRSS2 expression Transmission upregulated virus virus receptor
[DOI] 10.3389/fimmu.2022.957224 PMC 바로가기

Hydroxypropyl-beta-cyclodextrin (HP-BCD) inhibits SARS-CoV-2 replication and virus-induced inflammatory cytokines

하이드록시프로필-베타-사이클로덱스트린(HP-BCD)은 SARS-CoV-2 복제 및 바이러스 유발 염증성 사이토카인을 억제합니다

Article

Published on 2022-09-012022-09-11
Journal: Antiviral Research
[Category] COVID19(2023년), SARS, 변종, 신약개발,

[키워드] accompanied ACE2 Affect affected Alter antiviral activity Beta Beta-cyclodextrin Calu Calu-3 Cell cell line cell lines cholesterol COVID-19 COVID-19 disease cyclodextrin Cytokines cytopathic effects demonstrated disease drug Drug repurposing evidenced excipient experimental models expression Human Hyperinflammatory syndrome immunomodulatory effect in vitro in vivo Inflammation Inflammatory cytokine Inflammatory response inhibit inhibited inhibiting investigated microbial products mitigate Monocytes Particles pathophysiology primary human cells Proinflammatory cytokine proinflammatory cytokines protein levels reducing reduction in Replication replication complexes required Respiratory system safety profile SARS-CoV-2 SARS-CoV-2 replication SARS-CoV-2 variant SARS-CoV-2 variants SARS-CoV2 selected selective significantly systemic manifestations the disease therapeutic therapeutic agent therapeutic strategy These data treating Treatment viral cytopathic effect virus virus virus replication Virus replication. virus RNA
[DOI] 10.1016/j.antiviral.2022.105373 PMC 바로가기 [Article Type] Article

Self-assembling short immunostimulatory duplex RNAs with broad spectrum antiviral activity

Research article

Published on 2022-08-242022-10-05
Journal: Molecular Therapy. Nucleic Acids
[Category] 치료제,

[키워드] acute respiratory syndrome antiviral activity antiviral response antiviral therapeutic antiviral therapeutics base base pairs blunt cell lines Characteristics coronavirus coronavirus disease COVID-19 described DNA double-stranded RNA dsRNAs groups highlight Hoogsteen G-G base pairing human lung IFN IFN-I IFN-III immunostimulant immunostimulatory immunostimulatory RNA independent induce Infection Influenza influenza A virus interferon lack lung MERS-CoV Middle East monophosphate motif MT: Oligonucleotides not affect organ on a chip pandemic pathophysiology pathway proinflammatory receptor respiratory syndrome coronavirus respiratory virus RIG-I RNA RNAs SARS-CoV SARS-CoV-2 SARS-COV-2 infection sequence siRNAs terminal hydroxyl Therapies and Applications TLR type I type III interferon unique
[DOI] 10.1016/j.omtn.2022.08.031 [Article Type] Research article

Selection of Bis-Indolyl Pyridines and Triphenylamines as New Inhibitors of SARS-CoV-2 Cellular Entry by Modulating the Spike Protein/ACE2 Interfaces

스파이크 단백질/ACE2 인터페이스를 조절하여 SARS-CoV-2 세포 진입의 새로운 억제제로서 비스-인돌릴 피리딘 및 트리페닐아민 선택

Article

Published on 2022-08-162022-09-11
Journal: Antimicrobial Agents and Chemotherapy
[Category] COVID19(2023년), SARS, 변종,

[키워드] 50% effective concentration ACE2 acute respiratory syndrome acute respiratory syndrome coronavirus approaches association biochemical caused Cell cell lines cellular cellular receptor circulating circulating variant complex Compound coronavirus coronavirus disease COVID COVID-19 docking drug functional human cell lines identify in silico indicate Infection infectious agent inhibit inhibitor inhibitory inhibitory effect Interaction interface Lentiviral vector lentiviral vectors modulating molecular molecules New pandemic parameter pseudotyped pyridine receptor ACE2 S protein SARS-CoV-2 SARS-CoV-2 entry SARS-CoV-2 S protein SARS-CoV-2 strain screened selected Selection severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 spike susceptible the SARS-CoV-2 the Spike therapeutic purposes these compound these compounds triphenylamine variant viral entry viral entry. viral infection viral replication virus wild-type
[DOI] 10.1128/aac.00083-22 PMC 바로가기 [Article Type] Article

SARS-CoV-2 nucleocapsid protein triggers hyperinflammation via protein-protein interaction-mediated intracellular Cl – accumulation in respiratory epithelium

SARS-CoV-2 뉴클레오캡시드 단백질은 단백질-단백질 상호작용 매개 세포내 Cl-호흡 상피에서의 축적을 통해 과염증을 유발합니다

Article

Published on 2022-07-272022-09-11
Journal: Signal Transduction and Targeted Therapy
[Category] COVID19(2023년), SARS, 신약개발, 치료법,

[키워드] abrogated Airway inflammation CAMP cell lines cells CFTR Concentration COVID-19 cystic fibrosis Cytokine storms Defense demonstrated depleting downregulated elicit epithelial epithelial cell epithelial cells expression fibrosis glucocorticoid Host host defense human airway epithelial hyperinflammation immune response immune responses Inflammatory response Inflammatory responses inhibition inhibitor Intracellular knockout microRNA modulating murine model murine models N protein pathogen phosphodiesterase Phosphorylation promoted Protein pulmonary inflammation raised reducing regulated respiratory epithelium resulting robust Rolipram SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 nucleocapsid second messenger selective serum SGK1 signaling pathway Smad3 structural protein suggested sustained targeting therapeutic strategy transmembrane Trigger upregulation
[DOI] 10.1038/s41392-022-01048-1 PMC 바로가기 [Article Type] Article